TruSeq Stranded mRNA

RNA sequencing Mouse - NSC-34

Experiment
RNA sequencing Mouse - NSC-34
Product
TruSeq Stranded mRNA from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
The protocol is optimized for 0.1–1 µg of total RNA

Publication protocol

Total and Polysomal RNA samples were converted to cDNA libraries according to the Illumina TruSeq Stranded mRNA Library Prep. The sequencing was performed on Illumina HiSeq 2500 platform.

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Reviews

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Discussion

Discussion

4 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - NSC-34 using TruSeq Stranded mRNA from Illumina.

Paper title
HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA
Full paper
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA sequencing Mouse - NSC-34 using TruSeq Stranded mRNA from Illumina. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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