TruSeq Stranded mRNA

RNA sequencing Mouse - 3T3-L1

Experiment
RNA sequencing Mouse - 3T3-L1
Product
TruSeq Stranded mRNA from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
The protocol is optimized for 0.1–1 μg of total RNA.

Publication protocol

Live, cultured cells (Flp-In T-Rex 293 HEK cells, NIH/3T3 cells), intact, live Drosophila melanogaster embryos and sorted, methanol-fixed cells from dissected newborn mouse hindbrain and cerebellum were used for total RNA extraction with TRIZOL. Strand-specific cDNA libraries were generated according to the Illumina TruSeq protocol (TruSeq Stranded mRNA LT Sample Prep Kit, Illumina) using between 24 to 260 ng of total RNA input. The 1.8-pM libraries were sequenced on an Illumina NextSeq 500 System, using the High Output v2 Kit (150 cycles), single read: 150 bp, index read: 6 bp.

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Discussion

Discussion

2 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - 3T3-L1 using TruSeq Stranded mRNA from Illumina.

Paper title
Cell fixation and preservation for droplet-based single-cell transcriptomics
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

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