TruSeq Stranded Total RNA

RNA sequencing Mouse - 3T3-L1

Experiment
RNA sequencing Mouse - 3T3-L1
Product
TruSeq Stranded Total RNA from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
The protocol is optimized for 0.1–1 μg of total RNA.

Publication protocol

RNA was extracted with TRIzol reagent (Ambion) and purified with the Direct-zol RNA MiniPrep kit (Zymo Research). DNase I treatment was performed on the Direct-zol column. RNA concentration was determined with a Nanodrop apparatus (Thermo Fisher Scientific) and its integrity (size distribution) with a Bioanalyzer 2100 (Agilent).
Libraries were prepared using TrueSeq stranded total RNA sample preparation (Illumina). Sequencing was performed on an Illumina HiSeq 2000 sequencer with 100 bp single-end reads. Reads were aligned with TopHat (v2.0.7) to the mouse genome (v10) or the human genome (v19). 7SL and RNase MRP reads were quantified using Bedtools (v2.15.0).

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Discussion

Discussion

2 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - 3T3-L1 using TruSeq Stranded Total RNA from Illumina.

Paper title
7SL RNA in vertebrate red blood cells
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded Total RNA below.

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