|Libraries were created from high quality RNA (RNA integrity number of 7 or greater) using the SENSE mRNA-Seq Library Prep Kit from Lexogen (Lexogen, Vienna, Austria) according to the manufacturer’s protocol. This protocol includes an integrated poly(A) mRNA selection process, and the constructed library maintains the strand specificity. One single RNA-Seq library was constructed for each RNA sample. Library production is initiated by the random hybridization of Starter/Stopper heterodimers to the RNA template, followed by first and second strand cDNA synthesis.|
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