KAPA Stranded RNA-Seq Kits with RiboErase (HMR)

RNA sequencing Rat - Lung tissue

Experiment
RNA sequencing Rat - Lung tissue
Product
KAPA Stranded RNA-Seq Kits with RiboErase (HMR) from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Protocol tips
This protocol has been validated for library construction from 100 ng – 1 µg total RNA, in 10 µL of RNase-free water.

Publication protocol

Three fragmented frozen lung tissue samples were randomly selected from each group for the RNA isolation in 3 replicates. Total RNAs were extracted using TRIzol™ Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions and random primers were used to generate complementary DNA. After quantitative analysis and quality inspection, KAPA-Stranded RNA-Seq Library Prep Kit (Illumina) was used to construct sequencing libraries. Sequencing was carried out using an Illumina HiSeq 4000 Sequencing System for 150 cycles. After the data preprocessing, gene level fragments per kilobase of exon per million fragments mapped and significant changes in gene and transcript expression were then calculated using Ballgown software.

Full paper   Login or join for free to view the full paper.

Reviews

KAPA Stranded RNA-Seq Kits with RiboErase (HMR) from Roche Lifesciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Rat - Lung tissue using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) from Roche Lifesciences.

Paper title
Candidate Genes as Biomarkers in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Based on mRNA Expression Profile by Next-Generation RNA-Seq Analysis
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Roche Lifesciences for KAPA Stranded RNA-Seq Kits with RiboErase (HMR) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA sequencing Rat - Lung tissue using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms