TruSeq Stranded mRNA

RNA sequencing Rat - Retinal ganglion cells (RGCs)

Experiment
RNA sequencing Rat - Retinal ganglion cells (RGCs)
Product
TruSeq Stranded mRNA from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
The protocol is optimized for 0.1–1 µg of total RNA.

Publication protocol

RNA samples were processed by the high-throughput Genomics group at the Wellcome Trust Centre for Human Genetics. Samples were amplified by SMARTer (ClonTech, CA, US) due to the low quantities of RNA extracted from individual stellate ganglia. Library preparation used total RNA with the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Samples were sequenced using an Illumina Hi-Seq 4000 (Illumina, CA, USA), with samples repeated across two lanes to reduce technical error and increase read depth. The samples were sequenced with a paired end protocol (75 base pairs).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Rat - Retinal ganglion cells (RGCs) using TruSeq Stranded mRNA from Illumina.

Paper title
Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

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