NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Protein isolation Mammalian cells - HLE-B3

Experiment
Protein isolation Mammalian cells - HLE-B3
Product
NE-PER™ Nuclear and Cytoplasmic Extraction Reagents from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Follow the manufacturer's protocol.

Publication protocol

Nuclear proteins were isolated from lenses by using commercially available nuclear and cytoplasmic extraction kits (NE-PER™ Nuclear and Cytoplasmic Extraction Reagents, Pierce, IL). NF-κB DNA-binding activity was evaluated using the ELISA-based EZ-detected™ Transcription Factor Kit for NF-κB p65 (Pierce, IL) according to the manufacturer's instructions. The amount of active NF-κB p65 in each sample was obtained from a standard curve and normalized to the protein content.

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Papers

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Paper title
Accumulation of argpyrimidine, a methylglyoxal-derived advanced glycation end product, increases apoptosis of lens epithelial cells both and
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for NE-PER™ Nuclear and Cytoplasmic Extraction Reagents below.

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