|The tissues were homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C.|
|Protein concentration was determined using the DC Protein Assay kit (Bio-Rad).|
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
1 year ago
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - ME epithelial tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.
Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.Download manufacturer protocol
Check out videos that might be relevant for performing Protein isolation Tissue - ME epithelial tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.