Total Exosome RNA & Protein Isolation Kit

Protein isolation Mammalian cells - HeLa

Experiment
Protein isolation Mammalian cells - HeLa
Product
Total Exosome RNA & Protein Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
The Total exosome RNA and protein isolation kit (Invitrogen) was utilized for recovery of RNA from the exosome samples obtained with the reagent and ultracentrifugation protocol and parental samples for each sample type, HeLa cell pellets (1 × 106 cells) and cell-free serum.

Publication protocol

The Total exosome RNA and protein isolation kit (Invitrogen) was utilized for recovery of RNA from the exosome samples obtained with the reagent and ultracentrifugation protocol and parental samples for each sample type, HeLa cell pellets (1 × 106 cells) and cell-free serum. 200 μL of each sample (brought up to volume with PBS if necessary) was combined with 205 μL of 2x denaturing solution, vortexed to lyse, and then incubated on ice for 5 min. After incubation, 410 μL of acid-phenol : chloroform was added to the mixture and vortexed for 30–60 sec to mix. Samples were then centrifuged for 5 min at 10,000 g at room temperature to separate the mixture into aqueous and organic phases. Once centrifugation was complete, the aqueous (upper) phase was carefully removed without disturbing the lower phase or the interphase and transferred to a fresh tube.

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Papers

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Paper title
The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Total Exosome RNA & Protein Isolation Kit below.

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