M-PER™ Mammalian Protein Extraction Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- M-PER containing Halt™ Protease and Phosphatase Inhibitor is added to the cell pellets and proteins are extracted by gentle shacking for 10 min followed by centrifugation at 13,000× g for 15 min.

Protocol tips
- M-PER containing Halt™ Protease and Phosphatase Inhibitor is added to the cell pellets and proteins are extracted by gentle shacking for 10 min followed by centrifugation at 13,000× g for 15 min.

Publication protocol

Following treatments, HAEC were washed twice with PBS, detached from the flasks by using trypsin-EDTA, centrifuged and stored at −20 °C. Mammalian Protein Extraction Reagent (M-PER, Thermo Fisher Scientific, Milano, Italy) containing Halt™ Protease and Phosphatase Inhibitor (Single-Use Cocktail, Thermo Scientific) was added to the cell pellets and proteins were extracted by gentle shacking for 10 min followed by centrifugation at 13,000× g for 15 min in order to remove cell debris. Protein quantification was determined according to the Bio-Rad Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA) using bovine serum albumin (BSA) as standard.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for M-PER™ Mammalian Protein Extraction Reagent below.

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