CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Mouse lung tissue

Experiment
Protein isolation Tissue - Mouse lung tissue
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- The tissues are homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C.

Publication protocol

Total protein extracted from the ME epithelial cells and lungs of two-months-old wild-type, Nischedsn/edsn and Itga5tm1Hyn/+; Nischedsn/edsn mice were used for the western blot analysis. Each middle ear sample consisted of combined epithelial cells scooped out of both ears of one mouse. Each lung sample consisted of whole lung tissue from one mouse. Either three or four biological replicates were performed for each antibody. The tissues were homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C. Protein concentration was determined using the DC Protein Assay kit (Bio-Rad).

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Discussion

Discussion

2 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse lung tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Paper title
A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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