RIPA Lysis Buffer (ProteinSimple)
Protein isolation Tissue - Mouse aorta
Publication protocol
The aortas obtained from mice in each groups were homogenized with 100 μL RIPA Lysis Buffer. The homogenate was placed on ice for 1 h then centrifuged at 15000 for 15 minutes at 4°C. The supernatant was collected and denatured in boiling water for 8 min. The protein samples were stored at −20°C until further analysis.Full paper Login or join for free to view the full paper.
Reviews
RIPA Lysis Buffer (ProteinSimple) from ProteinSimple has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
4 years ago
Author:
When should I be harvesting the tissue before using it for the most optimal results?
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse aorta using RIPA Lysis Buffer (ProteinSimple) from ProteinSimple.
Manufacturer protocol
Download the product protocol from ProteinSimple for RIPA Lysis Buffer (ProteinSimple) below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Protein isolation Tissue - Mouse aorta using RIPA Lysis Buffer (ProteinSimple) from ProteinSimple. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment