CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Human umbilical cord tissue

Experiment
Protein isolation Tissue - Human umbilical cord tissue
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Protease inhibitor is added to the lysis buffer.

Publication protocol

Snap-frozen UCT was segmented into pieces weighing approximately 30 mg and thawed in a 200 ul solution of tissue lysis buffer (Sigma, #C3228) with 5 ul/ml protease inhibitor (Sigma, #P8340). Thawed samples were homogenized and spun at 16,000xg for 10 minutes. The supernatant was collected and stored at −80°C until protein analysis.

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Discussion

Discussion

2 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Human umbilical cord tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Paper title
Infant Adiposity is Independently Associated with a Maternal High Fat Diet but Not Related to Niacin Intake: The Healthy Start Study
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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Videos

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