N788 phenol-free total RNA purification kit
RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa
Protocol tips
Publication protocol
RNA extraction was done with cell pellets containing 1 × 109 cells from three independent biological replicates of each condition using three different kits/reagent: TRI Reagent, RNeasy Mini kit and Phenol-free total RNA purification kit.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
Some help with RNA isolation using Trizol
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
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Manufacturer protocol
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