Tissue or Cell Total Protein Extraction Kit

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	Total proteins of five muscle tissues were extracted by using a Total Protein Extraction Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s protocol, and then the muscle proteins were preserved at −80 °C in a freezer.

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Total proteins of five muscle tissues were extracted by using a Total Protein Extraction Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s protocol, and then the muscle proteins were preserved at −80 °C in a freezer.

Publication protocol

Total proteins of five muscle tissues were extracted by using a Total Protein Extraction Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s protocol, and then the muscle proteins were preserved at −80 °C in a freezer. Western blotting was used to analyze the relative protein expression of PPP3CA in muscle tissues of Tianfu goat at 150 days old. The determination of the protein concentration used BCA protein assay kit (Beyotime, Shanghai, China) with bovine serum albumin (BSA) as a standard. GAPDH protein was selected as an internal control. Polyclonal antibodies to the calcineurin protein (Abcam, Cambridge, UK) were used at 1/500 dilution and a monoclonal antibody to the GAPDH protein (BiYunTian, Shanghai, China) was at a 1/1000 dilution. Equal amounts of protein (12 μg per lane) were resolved on 12% SDS-polyacrylamide gels, and then as detailed previously in [33], the intensity of the signal was used to analyze the relative amount of PPP3CA protein in Tianfu goat muscle tissues extracts.

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