Tissue or Cell Total Protein Extraction Kit

Protein isolation Tissue - Lamb muscle tissue

Experiment
Protein isolation Tissue - Lamb muscle tissue
Product
Tissue or Cell Total Protein Extraction Kit from Sangon Biotech
Manufacturer
Sangon Biotech

Protocol tips

Protocol tips
Total proteins of five muscle tissues were extracted by using a Total Protein Extraction Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s protocol, and then the muscle proteins were preserved at −80 °C in a freezer.

Publication protocol

Total proteins of five muscle tissues were extracted by using a Total Protein Extraction Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s protocol, and then the muscle proteins were preserved at −80 °C in a freezer. Western blotting was used to analyze the relative protein expression of PPP3CA in muscle tissues of Tianfu goat at 150 days old. The determination of the protein concentration used BCA protein assay kit (Beyotime, Shanghai, China) with bovine serum albumin (BSA) as a standard. GAPDH protein was selected as an internal control. Polyclonal antibodies to the calcineurin protein (Abcam, Cambridge, UK) were used at 1/500 dilution and a monoclonal antibody to the GAPDH protein (BiYunTian, Shanghai, China) was at a 1/1000 dilution. Equal amounts of protein (12 μg per lane) were resolved on 12% SDS-polyacrylamide gels, and then as detailed previously in [33], the intensity of the signal was used to analyze the relative amount of PPP3CA protein in Tianfu goat muscle tissues extracts.

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Discussion

Discussion

2 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Lamb muscle tissue using Tissue or Cell Total Protein Extraction Kit from Sangon Biotech.

Paper title
Molecular Cloning, Structural Analysis and Tissue Expression of () Gene in Tianfu Goat Muscle
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Manufacturer protocol

Download the product protocol from Sangon Biotech for Tissue or Cell Total Protein Extraction Kit below.

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