CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Mouse heart

Experiment
Protein isolation Tissue - Mouse heart
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- The frozen tissues are homogenized in CelLytic™ MT cell lysis reagent by Precellys 24 beads homogenizer. The homogenates are centrifuged and the supernatants are used.

Publication protocol

C57/BL6 mice were administrated either atorvastatin (10 mg/kg per day), pravastatin (50 mg/kg per day), or placebo by gavage every day for 1 week. After the 1-week treatment, the animals were anaesthetized with isofluorane and perfused with ice-cold PBS. Immediately after dissection, the aorta, heart, liver, and skeletal muscles were frozen by liquid nitric oxide. The frozen tissues were homogenized in CelLytic™ MT cell lysis reagent (Sigma, C3228) by Precellys 24 beads homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France). The homogenates were centrifuged and the supernatants were used for western blot analysis.

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Discussion

Discussion

1 year ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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