CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Rat skin tissue

Experiment
Protein isolation Tissue - Rat skin tissue
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Proteins are extracted in CelLytic™ MT Cell Lysis Reagent mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads.

Publication protocol

Tissue was harvested at 2 and 5 weeks after PEF treatment, immediately flash frozen in liquid nitrogen and kept at −80 °C. The center of the treated area was excised and proteins were extracted in CelLytic™ MT Cell Lysis Reagent, C3228 (Sigma, MO) mixed with Protease Inhibitor Cocktail P8340 (Sigma, MO) using Mini-Beadbeater-1 (Biospec, OK) with 5.5 g/cc density zirconia beads (Biospec, OK). Tubes with the buffer and beads were shakes 4 times for 15 seconds with 1-minute intervals, when the tubes were kept on ice. Immediately after the extraction, total protein was quantified using 660 nm Protein Assay (Pierce,IL).

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Discussion

Discussion

2 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Rat skin tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Paper title
Skin Rejuvenation with Non-Invasive Pulsed Electric Fields
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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