Direct-zol™ RNA MiniPrep Plus

RNA isolation / purification Bacteria - Gram negative Escherichia coli

Experiment
RNA isolation / purification Bacteria - Gram negative Escherichia coli
Product
Direct-zol™ RNA MiniPrep Plus from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
Tough-to-lyse samples (i.e.,gram-positive bacteria, yeast, fungi, animal tissue, plant, etc.) can be homogenized in TRI Reagent® (supplied with kit) with a mortar/pestle, dounce, tissue grinder, or bead beating with a high-speed homogenizer prior to RNA Purification.

Publication protocol

RNAs for primer extension were isolated using Direct-zol RNA MiniPrep according to the manufacturer’s instructions

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

Some help with RNA isolation using Trizol

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

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Papers

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Manufacturer protocol

Download the product protocol from Zymo Research for Direct-zol™ RNA MiniPrep Plus below.

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