CelLytic™ Y Plus Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Yeast cells expressing Msg on the surface were treated with β-1,3 glucanase (lyticase, CelLytic Y Plus Kit, Sigma-Aldrich) overnight at 37°C.

Protocol tips
Yeast cells expressing Msg on the surface were treated with β-1,3 glucanase (lyticase, CelLytic Y Plus Kit, Sigma-Aldrich) overnight at 37°C.

Publication protocol

Yeast cells expressing Msg on the surface were treated with β-1,3 glucanase (lyticase, CelLytic Y Plus Kit, Sigma-Aldrich) overnight at 37°C. The supernatant obtained was analyzed by Western blot, and the pellet was used for immunofluorescence analysis. In some experiments, yeast cells expressing Msg were broken with glass beads to prepare cell extracts for Western blot analysis.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Yeast - Saccharomyces cerevisiae using CelLytic™ Y Plus Kit from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ Y Plus Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Yeast - Saccharomyces cerevisiae using CelLytic™ Y Plus Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.