Y-PER™ Yeast Protein Extraction Reagent

Protein isolation Yeast - Candida tenuis

Experiment
Protein isolation Yeast - Candida tenuis
Product
Y-PER™ Yeast Protein Extraction Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- The extraction was performed according to the manufacturer's protocol.

Publication protocol

The enzymatic activity of xylose reductase (XR) and xylitol dehydrogenase (XDH) in crude-cell extracts was measured according to [23]. For this, 5 mL of cell suspension of S. stipitis, S. passalidarum, S. arborariae and C. tenuis were collected in the middle of the exponential growth phase during aerobic and oxygen-limited fermentations. Cells were pelleted by centrifugation, washed with sterile water, and lysed with Y-PER®—Yeast Protein Extraction Reagent (Pierce, Rockford, USA) to obtain cell-crude extracts. Protein concentrations in cell-free extracts were determined using Quick StartTM Bradford Protein Assay Kit (Bio-Rad Laboratories Ltda., USA), following the manufacture’s instruction.

Full paper   Login or join for free to view the full paper.

Reviews

Y-PER™ Yeast Protein Extraction Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Yeast - Candida tenuis using Y-PER™ Yeast Protein Extraction Reagent from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Y-PER™ Yeast Protein Extraction Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Yeast - Candida tenuis using Y-PER™ Yeast Protein Extraction Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms