Gentra Puregene Blood Kit

DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines

Experiment
DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines
Product
Gentra Puregene Blood Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Do not incubate samples with lysis buffer overnight at 37°C. 2-3 hrs is enough

Publication protocol

DNA extraction and freeze drying
Cells were bulk cultured (ECACC, Porton Down, UK) to approximately 1010 cells and frozen as pellets of 108 cells. Genomic DNA was extracted from cell pellets using the PureGene DNA purification and Autopure LS equipment (Qiagen, Crawley, UK). The integrity of the extracted DNA was confirmed by electrophoresis in agarose gels. Each of the DNA samples was dissolved at a concentration of approximately 29 μg/ml in 0.8 m Tris/0.08 m EDTA buffer with 2.5 mg/ml Trehalose. A volume of 0.8 ml of this solution was dispensed into a minimum of 1200 glass ampoules and freeze-dried. Sealed ampoules were stored at −20 °C.

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Discussion

Discussion

4 years ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines using Gentra Puregene Blood Kit from Qiagen.

Paper title
Preparation and validation of the first WHO international genetic reference panel for Fragile X syndrome
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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Blood Kit below.

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Videos

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