Gentra Puregene Blood Kit

DNA isolation / purification Cells - Primary cells CD4+ T cells

Experiment
DNA isolation / purification Cells - Primary cells CD4+ T cells
Product
Gentra Puregene Blood Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Frozen samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Publication protocol

Genomic DNA and RNA isolation.
Magnetic bead depletion was performed on PBMCs to enrich for resting CD4 T cells as described previously (). Genomic DNA was purified from resting CD4 T cells using the Puregene kit (Gentra). 10–20 ml of plasma from each patient was ultracentrifuged at 25,200 for 2 h at 4° to concentrate plasma virions. Viral RNA was isolated from pelleted virions using the QIAGEN Viral RNA isolation kit (QIAGEN). In brief, supernatant was removed after centrifugation, and pelleted virions were resuspended in the supplied lysis buffer. RNA was isolated according to the recommended protocol.

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Discussion

Discussion

4 years ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - Primary cells CD4+ T cells using Gentra Puregene Blood Kit from Qiagen.

Paper title
Maintenance of viral suppression in HIV-1–infected HLA-B*57 elite suppressors despite CTL escape mutations
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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Blood Kit below.

Download PDF Download manufacturer protocol

Videos

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