TRI Reagent® Sigma

RNA isolation / purification Cells - immortalized A2780ADR

Experiment
RNA isolation / purification Cells - immortalized A2780ADR
Product
TRI Reagent® Sigma from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA.

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

RNA from cells was extracted using Tri-Reagent (Sigma), as per manufacturer’s instructions.

The RNA isolated was then treated with DNase (Promega) before undergoing reverse transcription by heating to 25°C for 10 min, 55°C for 50 min and 70°C for 15 min. After this, cDNA samples were stored at −20°C.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized A2780ADR using TRI Reagent® Sigma from Sigma-Aldrich.

Paper title
Secreted frizzled-related protein 4 expression is positively associated with responsiveness to Cisplatin of ovarian cancer cell lines and with lower tumour grade in mucinous ovarian cancers
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

Download PDF Download manufacturer protocol

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