NucleoSpin® RNA

RNA isolation / purification Cells - immortalized BxPC-3

Experiment
RNA isolation / purification Cells - immortalized BxPC-3
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Quadruplicates samples of 6×104 cells (BxPC-3, MIA PACA-2 and CHO-WT) were seeded in 12-well plates and initially cultured for 48 h. Cell total-RNA was extracted using Nucleospin RNA II (Macherey-Nagel, Duren, Germany) with on-column DNA digestion, according to manufacturer's instructions. TRIzol LS (Life Technologies Corporation, Carlsbad, CA, USA) was used for the homogenization of the human tissue sample in a Mini-BeadBeater-1 (Biospec Products, Coventry, UK). The RNA-containing aqueous phase was isolated after Chloroform phase separation followed by an Isopropanol nucleic acid-precipitation step. The resulting RNA pellet was further processed with the Nucleospin RNA II kit as above. RNA eluates' concentration and purity were determined with NanoDrop 2000 (Thermoscientific, Open Biosystems, Lafayette, CO, USA).

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized BxPC-3 using NucleoSpin® RNA from Macherey Nagel.

Paper title
Microcystin LR Shows Cytotoxic Activity Against Pancreatic Cancer Cells Expressing the Membrane OATP1B1 and OATP1B3 Transporters.
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Manufacturer protocol

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