TRIzol Reagent

RNA isolation / purification Cells - immortalized Cal-27

Experiment
RNA isolation / purification Cells - immortalized Cal-27
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

The tissue sample was grinded in pre-chilled mortars with liquid nitrogen. TRIzol reagent (1 ml per 50-100 mg) was added when homogenizing. Then, the powders were transferred to 2-ml or 1.5-ml microcentrifuge tubes. The cultured cells were lysed directly in the dish with 0.3-0.4 ml of TRIzol reagent per 1 × 105-107 cells. Then, RNA was isolated from harvested cells, xenograft tumors, or human tissues with TRIzol reagent according to the manufacturer’s instructions (Invitrogen, CA, USA). Real-time PCR reactions were performed using SYBR Premix DimerEraser (Takara, Dalian, China), and human GAPDH was used as an endogenous control for mRNA detection. The expression of each gene was quantified by measuring Ct values and normalized using the 2-ΔΔct method relative to GAPDH

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Cal-27 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Upregulation of the long non-coding RNA affects the proliferation, invasion and survival of tongue squamous cell carcinoma via the Wnt/β-catenin signaling pathway
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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