TRIzol Reagent

RNA isolation / purification Cells - immortalized Calu-3

Experiment
RNA isolation / purification Cells - immortalized Calu-3
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Calu-3 cells were treated with 30nM Silencer Select negative control siRNA #2 or EHF siRNA (s25399, both from Ambion) using RNAiMax transfection reagent (LT). 48 h post-transfection, RNA was isolated from five samples per treatment using Trizol (LT). RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation Kit v2 per the manufacturer's Low-Throughput protocol (Illumina). The libraries were sequenced on Illumina HiSeq2500 machines. Data were analysed using TopHat and Cufflinks (30). qRT-PCR was performed with TaqMan Reverse Transcription reagents kit (LT), primer pairs specific for each target (Supplementary Table S2) and SYBR green

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

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