RNeasy Mini Kit

RNA isolation / purification Cells - immortalized COV-434

Experiment
RNA isolation / purification Cells - immortalized COV-434
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA from cells and tumor tissues were extracted using RNeasy Micro or Mini Kit (Qiagen) according to the manufacturer’s instructions cDNA synthesis from total RNA was performed using High Capacity RNA to cDNA Master Mix (Applied Biosystems) in accordance with the manufactures instructions. Real time quantitative PCR was performed using an Applied Biosystems Prism 7500 or Step-One Plus sequence detection system and data were analyzed according to the ΔΔCT method and expressed relative to the control mean (set to equal “1”) as described previously

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Reviews

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized COV-434 using RNeasy Mini Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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