TRI Reagent™ Solution

RNA isolation / purification Cells - immortalized Ku812

Experiment
RNA isolation / purification Cells - immortalized Ku812
Product
TRI Reagent™ Solution from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA.

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA isolation was performed by cell lysis with TRI-Reagent (Ambion, Foster City, USA) by taking the upper aqueous phase obtained after centrifugation at 1000g for 10 min. RNA was then washed in isopropanol and cool 75% ethanol, resuspended in nuclease-free water and kept at -20°C for further analysis.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRI Reagent™ Solution below.

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