RNeasy Micro Kit

RNA isolation / purification Cells - immortalized Ku812

Experiment
RNA isolation / purification Cells - immortalized Ku812
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

KU812 cells transduced with a random (RDM) short hairpin RNA (shRNA) or a CD25 shRNA were subjected to RNA isolation and gene array analysis using Affymetrix technology.

Total RNA was extracted from CML cells using RNeasy Micro-Kit (Qiagen) and used (150 ng total RNA) for Gene Chip analyses. Preparation of terminal-labeled cRNA, hybridization to genome-wide human Gene 2.0 ST Arrays (Affymetrix, Santa Clara, CA, USA) and scanning of arrays were carried out according to the manufacturer's protocols (https://www.affymetrix.com). Robust Multichip Average (RMA) signal extraction and normalization were performed using GeneChip Expression Console Version 1.3.1. Differences in mRNA expression levels were calculated as normalized fluorescence signal ratio of KU812 CD25 shRNA cells versus KU812 RDM shRNA cells.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Ku812 using RNeasy Micro Kit from Qiagen.

Paper title
Identification of CD25 as STAT5-Dependent Growth-Regulator of Leukemic Stem Cells in Ph+ CML
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

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