TRIzol Reagent

RNA isolation / purification Cells - immortalized MDA-MB-361

Experiment
RNA isolation / purification Cells - immortalized MDA-MB-361
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Cell line total RNA was extracted using the TRIzol® method (Life Technologies, USA) after lysis of subconfluent cultures in 25cm2 culture flasks (T25). As mentioned above, yield and purity of the isolates were tested using a NanoDrop® system and stored at −80°C.

From every clinical sample, 6-12 10μm-sections were made and stored in cryotubes (−80°C) for RNA-extraction. RNA was extracted from the samples using the RNeasy Lipid Tissue Mini Kit (Qiagen, Germany).

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

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