TRI Reagent® Sigma

RNA isolation / purification Cells - immortalized NTERA-2

Experiment
RNA isolation / purification Cells - immortalized NTERA-2
Product
TRI Reagent® Sigma from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA.

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was extracted from NT2 cells treated with chloroform extract of Rhazya stricta stems (RS1S CHCL3), RA, and vehicle control using Tri-reagent (Sigma) as described by Chomczynski and Mackey [25]. One microgram of RNA was used for cDNA synthesis according to the manufacturer’s instructions (Reverse Transcription System, Promega). PCR amplification reactions were performed in a total volume of 25 μl using GoTaq® Green Master Mix (Promega).

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized NTERA-2 using TRI Reagent® Sigma from Sigma-Aldrich.

Paper title
In vitro induction of human embryonal carcinoma differentiation by a crude extract of
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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