TRIzol Reagent

RNA isolation / purification Cells - immortalized PANC-1

Experiment
RNA isolation / purification Cells - immortalized PANC-1
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

For RT-PCR analysis in Bosc23 and PANC1 cells, RNA was isolated by using TriZol reagent (Invitrogen) and cDNA was generated with the First-Strand cDNA Synthesis Kit (Fermentas).

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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