TRIzol Reagent

RNA isolation / purification Cells - immortalized PC-3

Experiment
RNA isolation / purification Cells - immortalized PC-3
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from cells using TRIzol reagent (Life Technologies #15596026) followed by chloroform extraction, isopropanol precipitation, and a 75% (v/v) ethanol wash. RNA samples (1 μg) were reverse-transcribed to cDNA using a High Capacity cDNA kit (Applied Biosystems #4368814). cDNA in 1 μl of the reaction mixture was amplified with PCR Master Mix (Life Technologies #K0171) and 10 μmol each of the sense and antisense primers. The thermal cycle profile was as follows: denaturation at 95 °C for 30 s, annealing at 55–61 °C for 30 s depending on the primers used, and extension at 72 °C for 90 s. Each PCR reaction was carried out for 35 cycles, and the PCR products were size fractionated on 1% agarose gel/GelGreen (1:10000) (Fisher Biotec #41005) and visualized under UV Trans illumination (FB Biotech).

Full paper   Login or join for free to view the full paper.

Reviews

TRIzol Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized PC-3 using TRIzol Reagent from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized PC-3 using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms