miRNeasy Mini kit

RNA isolation / purification Cells - immortalized PNT2

Experiment
RNA isolation / purification Cells - immortalized PNT2
Product
miRNeasy Mini kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step

Publication protocol

Total RNA from cells and EV subpopulations was isolated using the miRNAeasy micro kit (Qiagen, Hilden, Germany), according to the manufacturer´s instructions. RNA samples were eluted in 14 μL of RNAse-free water, aliquoted, and stored at -80 °C. The quality and sized of the isolated RNA was measured by capillary electrophoresis using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). RNA from each sample was denatured at 72 °C for 2 min and loaded into RNA 6000 Nano and Pico total RNA kits (Agilent Technologies) to analyze RNA profile and concentration. The RNA concentration was used as a normalizing loading factor for all EV samples.

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized PNT2 using miRNeasy Mini kit from Qiagen.

Paper title
Distinct prostate cancer-related mRNA cargo in extracellular vesicle subsets from prostate cell lines
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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download PDF Download manufacturer protocol

Videos

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