|- To reduce RNA degradation do not wash cells before the addition of RNAzol® RT.
- To reduce DNA contamination, use sufficient amount of RNAzol® RT based on the area of the culture dish and not on cell number.
|To reduce DNA contamination extend the incubation time to 15 minutes before sedimentation.|
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1 year ago
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
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