SV Total RNA Isolation System

RNA isolation / purification Cells - immortalized Saos-2

Experiment
RNA isolation / purification Cells - immortalized Saos-2
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
- For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

- Do not freeze-thaw the DNase more than three times after rehydration

Publication protocol

RNA was isolated from cell lines using the SV Total RNA Isolation System (Promega). 1 to 1.5 μg of total DNA was converted to cDNA using the QuantiTech Reverse Transcription Kit (Qiagen) or Maxima First Strand cDNA Synthesis Kit (Fermenta Life Science) according to the manufacturer’s instruction.

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Reviews

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Saos-2 using SV Total RNA Isolation System from Promega.

Paper title
Inactivation of the Alternative Promoter B Is Associated with DNA Methylation and Histone Modification in Osteosarcoma Cell Lines U2OS and SaOS-2
Full paper
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download PDF Download manufacturer protocol

Videos

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