RNeasy Mini Kit

RNA isolation / purification Cells - immortalized Saos-2

Experiment
RNA isolation / purification Cells - immortalized Saos-2
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).

Publication protocol

Total RNA was isolated from MDA-468 and SaOs-2 cells using RNeasy RNA isolation kit (Qiagen, Germantown, USA) and applied for the complementary DNA synthesis using stem–loop reverse transcription primers.

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Reviews

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Saos-2 using RNeasy Mini Kit from Qiagen.

Paper title
Short stretches of rare codons regulate translation of the transcription factor ZEB2 in cancer cells
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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