TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

The Caco-2 and WiDr cell lines highly expressed hCG, and hCG mRNA from these two cell lines was used to generate cDNA for transfection into the three low hCG-expressing cell lines LoVo, HCA7, and T84. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA). Reverse transcription with random hexamer primers was performed with an Ominscript RT kit (Qiagen, Valencia, CA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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