RNeasy Protect Cell Mini Kit

RNA isolation / purification Cells - immortalized C6/36

Experiment
RNA isolation / purification Cells - immortalized C6/36
Product
RNeasy Protect Cell Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Detached cells were transferred into a sterile 2 ml tube and were stored immediately at −80°C until total RNA extraction. Cells were homogenized in 350 µl RLT buffer in QIAshredder spin columns (Qiagen, Hilden, Germany) prior to total RNA extraction with Qiagen RNeasy Protect cell mini kit (Qiagen) according to manufacturer's instructions.

Publication protocol

Upon infection, C6/36 cells were harvested at 0 min, 15 mins, 30 mins and 120 mins post infection (pi). At 0 min pi, cells were harvested immediately upon virus inoculation. At each time point, C6/36 cells were washed with 2 ml of the pre-warmed (28°C) maintenance medium. After decanting the maintenance medium, 1 ml of Qiagen Cell Protect solution was added to each flask. Detached cells were transferred into a sterile 2 ml tube and were stored immediately at −80°C until total RNA extraction. Cells were homogenized in 350 µl RLT buffer in QIAshredder spin columns (Qiagen, Hilden, Germany) prior to total RNA extraction with Qiagen RNeasy Protect cell mini kit (Qiagen) according to manufacturer's instructions.

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C6/36 using RNeasy Protect Cell Mini Kit from Qiagen.

Paper title
Mosquito Cellular Factors and Functions in Mediating the Infectious entry of Chikungunya Virus
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Protect Cell Mini Kit below.

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