RNeasy Mini Kit

RNA isolation / purification Cells - immortalized High Five

Experiment
RNA isolation / purification Cells - immortalized High Five
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted with QIAGEN RNeasy Mini Kit (QIAGEN, 74104) immediately after cell collection. RNA quality was examined using Agilent 2100 RNA chip (standard setting). Poly-A tailed RNA was enriched and used to construct sequencing library with Illumina TruSeq RNA Sample Prep Kit (Illumina, RS-122-2001), following standard instruction. RNA-seq library was sequenced on Illumina HiSeq 2000 platform.

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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