RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	At the appropriate timepoint(s), total RNA was harvested according to the manufacturer’s instructions (RNeasy Mini Kit; Qiagen, Valencia, CA).

Protocol tips
At the appropriate timepoint(s), total RNA was harvested according to the manufacturer’s instructions (RNeasy Mini Kit; Qiagen, Valencia, CA).

Publication protocol

Cells were seeded at similar cell densities in 6-well plates as described for Alcian Blue, Alizarin Red and Oil Red O staining experiments. At the appropriate timepoint(s), total RNA was harvested according to the manufacturer’s instructions (RNeasy Mini Kit; Qiagen, Valencia, CA). Reverse transcription into cDNA was performed according to the manufacturer’s instructions (Omniscript RT Kit; Qiagen, Valencia, CA). Semi-quantitative PCR was performed for target genes using Platinum blue mastermix as per manufacturer’s instructions (Life Technologies, Carlsbad, CA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C3H-10T1/2 using RNeasy Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized C3H-10T1/2 using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.