RNeasy Mini Kit

RNA isolation / purification Cells - immortalized CT26

Experiment
RNA isolation / purification Cells - immortalized CT26
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from CT26 cells using RNeasy Mini Kit (Qiagen, Dusseldorf, Germany). cDNAs were synthesized using PrimeScript™ RT reagent Kit (Takara, Dalian, China) and real-time PCR was performed using SYBR® Select Master Mix (Applied Biosystems, Foster, CA, USA) on an ABI 7500 series PCR machine (Applied Biosystems).

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CT26 using RNeasy Mini Kit from Qiagen.

Paper title
Mast Cell Targeted Chimeric Toxin Can Be Developed as an Adjunctive Therapy in Colon Cancer Treatment
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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