Absolutely RNA Microprep Kit

RNA isolation / purification Cells - immortalized EL4

Experiment
RNA isolation / purification Cells - immortalized EL4
Product
Absolutely RNA Microprep Kit from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Upstream tips
- This kit is designed for small amounts of cells (max 10^5).
Protocol tips
- For RNA to dissolve better and to yeild high level preheat elution buffer at 60C.
Downstream tips
- Because this kit is designed for low amounts of starting material, the total RNA yield can be low and might be hard to measure using a Nanodrop. Use a fluorescence-based system to quantify.

Publication protocol

Total RNA from cell lines, primary cells and mouse tumor tissue was extracted using
TRIzol (Invitrogen), RiboPureTM Blood kit (Invitrogen), RNeasy Mini kit (Qiagen) or
Absolutely RNA Nanoprep Kit (Stratagene). Total RNA (1-5 μg) was reverse-transcribed
using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City,
CA) or Superscript III (Life Technologies) with oligo (dT) 20 primer

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Absolutely RNA Microprep Kit below.

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