NucleoSpin® RNA

RNA isolation / purification Cells - immortalized MC-38

Experiment
RNA isolation / purification Cells - immortalized MC-38
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Total RNA from cultured MC38 cells was isolated using Nucleospin RNA isolation kit (Mecherey-Nagel) and reverse-transcribed with RevertAid First Strand cDNA Synthesis Kit (ThermoFisher) according to manufacturer instruction.

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Reviews

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MC-38 using NucleoSpin® RNA from Macherey Nagel.

Paper title
Intratumoral Lentivector-Mediated TGF-β1 Gene Downregulation As a Potent Strategy for Enhancing the Antitumor Effect of Therapy Composed of Cyclophosphamide and Dendritic Cells
Full paper
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

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Videos

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