TriPure Isolation Reagent

RNA isolation / purification Cells - immortalized RIN-5F

Experiment
RNA isolation / purification Cells - immortalized RIN-5F
Product
TriPure Isolation Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- For low contamination rate carefully remove the upper aqueous phase .

- Adding too little reagent can lead to contamination with DNA.

Publication protocol

For RT-PCR analysis, 80-90% confluent RIN-5F cells were starved in serum-free RPMI-1640 for 4 h at 37°C and stimulated with insulin, ZnSO4, or [Zn(hkt)2] for 2 h in
high glucose (25 mM) DMEM. After stimulation, total RNA was obtained using a TriPure Isolation Reagent (Sigma-Aldrich, St. Louis, MO, USA). Extracted RNA was
measured using a NanoDrop and purified using a DNase I Amplification Grade kit (Invitrogen, Carlsbad, CA, USA

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized RIN-5F using TriPure Isolation Reagent from Sigma-Aldrich.

Paper title
Anti-hyperglycemic Effect of Long-Term Bis(hinokitiolato)zinc Complex ([Zn(hkt)]) Ingestion on Insulin Resistance and Pancreatic Islet Cells Protection in Type 2 Diabetic KK-A Mice.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

Download PDF Download manufacturer protocol

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