QIAamp DNA Mini Kit

DNA isolation / purification Cells - immortalized cell lines renal cortical tubule epithelial cells

Experiment
DNA isolation / purification Cells - immortalized cell lines renal cortical tubule epithelial cells
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

For detection of SV40 DNA integration into the cell genome, DNA was isolated, using a commercial kit (QIAmp DNA mini kit, Qiagen). SV40 DNA was amplified by PCR, using specific oligonucleotide primers (Table 2).

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Reviews

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Discussion

Discussion

1 year ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - immortalized cell lines renal cortical tubule epithelial cells using QIAamp DNA Mini Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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