Aurum™ Total RNA Fatty and Fibrous Tissue Kit

RNA isolation / purification Tissue - Human Adipose

Experiment

RNA isolation / purification Tissue - Human Adipose

Product

Aurum™ Total RNA Fatty and Fibrous Tissue Kit from Bio-Rad Laboratories

Manufacturer

Bio-Rad Laboratories

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
It is recommended to put fresh tissue in PureZOL (or similar product) after dissection, or snap freeze in liquid nitrogen. For frozen tissue, use of a homogenizer is recommended for further processing.	Preheat the elution solution to 70°C in water bath prior to the elution step.

Upstream tips
It is recommended to put fresh tissue in PureZOL (or similar product) after dissection, or snap freeze in liquid nitrogen. For frozen tissue, use of a homogenizer is recommended for further processing.
Protocol tips
Preheat the elution solution to 70°C in water bath prior to the elution step.

Publication protocol

The total mRNAwas extracted from ~100 mg of frozen adipose tissue samples using Aurum™ Total RNA Fatty Acid and Fibrous Tissue Kit (Bio-Rad, Hercules, California) as suggested by manufacturer. The quality of the extracted mRNA was assessed spectrophotometrically using the A260/A280 ratio that in all samples was between 1.8 and 2.0. In addition, the presence of intact RNA was also confirmed electrophoretically at 1% agarose gels with 28S and 18S rRNA bands clearly visible and sharp.

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Adipose using Aurum™ Total RNA Fatty and Fibrous Tissue Kit from Bio-Rad Laboratories.

Paper title

Molecular signature of adipose tissue in patients with both Non-Alcoholic Fatty Liver Disease (NAFLD) and Polycystic Ovarian Syndrome (PCOS)

Full paper

Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aurum™ Total RNA Fatty and Fibrous Tissue Kit below.

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