GenElute™ Bacterial Genomic DNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Examine reagents for precipitation. If any reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves and cool to room temperature before use.

Upstream tips
Examine reagents for precipitation. If any reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves and cool to room temperature before use.

Publication protocol

Genomic analysis
Bacterial DNA was extracted from 1-day old cultures of selected strains using GenElute™ bacterial genome kit (Sigma Aldrich®, USA). The genome sequences were generated on an Illumina MiSeq using v3 chemistry and 300 bp paired end reads using dual indexed Nextera XT libraries. The de novo assembly was performed using SPAdes assembly program and Quast [32]. Genome annotation used the SEED-based automated annotation system provided by the RAST server (http://rast.nmpdr.org) and prokaryotic genome annotation system PROKKA [33].

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for GenElute™ Bacterial Genomic DNA Kit below.

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