AllPrep DNA/RNA Mini Kit

RNA isolation / purification Tissue - Human Bronchi

Experiment
RNA isolation / purification Tissue - Human Bronchi
Product
AllPrep DNA/RNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- Complete disruption of plasma membranes of cells and organelles is absolutely required to release all the nucleic acids contained in the sample.

- Different samples require different methods to achieve complete disruption.

- Incomplete disruption results in significantly reduced nucleic acid yields.

- Overloading the spin columns significantly reduces nucleic acid yields.
Protocol tips
- Homogenizing the material is necessary to redice the viscosity of the lysates caused by cell disruption.

- Incomplete homogenization results in inefficient binding of DNA and RNA and therefore significantly reduced yield and purity of nucleic acids.

- Excessive homogenization, on the other hand, results in shorter genomic DNA fragments.

- The centrifugation temperature should be 20–25ºC.

-Warm the lysate to 37ºC before transferring it to the AllPrep DNA spin column.

Publication protocol

Allprep DNA/RNA mini kit (Qiagen, Germany) was used to extract and purify the RNA and DNA simultaneously. It is a technique of collecting data throughout the PCR process as it occurs, thus combining amplification and detection into a single step. RT-PCR even needs less RNA to run the gene expression analysis. In our study, we performed the RNA quantification and quality analysis with Nanodrop-2000. RNA and DNA were stored at −80°C for future biomarker analysis.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Bronchi using AllPrep DNA/RNA Mini Kit from Qiagen.

Paper title
EBUS-TBNA Provides Highest RNA Yield for Multiple Biomarker Testing from Routinely Obtained Small Biopsies in Non-Small Cell Lung Cancer Patients - A Comparative Study of Three Different Minimal Invasive Sampling Methods
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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