MagNA Pure Compact RNA Isolation Kit
RNA isolation / purification Tissue - Human Bronchi
Publication protocol
The uMS pellet and tissue from the core were used to obtain DNA or RNA. These samples were homogenized with a MagNA Lyser for 60 seconds at 6500 rpm and placed on a cooling block for 1 minute. Supernatants (100 μl) were used for DNA extraction with a MagNA Pure Compact Nucleic Acid Isolation Kit. Supernatants (350 μl) from homogenates were centrifuged and RNA was extracted using a MagNA Pure Compact RNA Isolation Kit. cDNA cloning was performed with a First Strand cDNA Synthesis Kit (Roche). Extracted DNA and cDNA were stored at 4°C.Full paper Login or join for free to view the full paper.
Reviews
MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Bronchi using MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences.
Manufacturer protocol
Download the product protocol from Roche Lifesciences for MagNA Pure Compact RNA Isolation Kit below.
Download manufacturer protocol
Videos
Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Bronchi using MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment